Development of Nano–Micro Fused LSPR Chip for In Situ Single-Cell Secretion Analysis

Single-cell analysis has become increasingly important in uncovering cell heterogeneity, which great implications medicine and biology for a deep understanding of characteristics. Owing to its significance, it is vital create novel devices that can reveal special or unique cells. In this work, we developed single-cell secretion detection chip consisting microwells trap single Each well surrounded by Au nanopillars capable localized surface plasmon resonance (LSPR) measurement. Using microfabrication nanofabrication techniques, nanopillar microwell structures were fabricated on COP film. The was modified with IL-6 antibodies the direct secreted via LSPR absorbance peak shift. Specific successfully demonstrated using null oversecreting Jurkat cell. A high trapping efficiency over 80% also achieved. Overall, development simple measurement setup represents significant field immunology, providing researchers powerful tool studying individual cells their cytokines, useful point-of-care testing (POCT) diagnostics.